YEAR OF IMPLEMENTATION: The Syllabus will be implemented from Academic Year 2016-17 onwards.
The Ph.D course work shall involve Four papers and Dissertation Viz.
i. Research Methodology
ii. Computer Application
iii. Review of Research Work
iv. Microbial Metabolism
v. Dissertation and Via-Voc
Ph.D. Course Work Syllabus
Introduction: Definition of Research, Qualities of Researcher, Components of Research Problem, Various Steps in Scientific Research, Types of Research; Hypotheses Research Purposes - Research Design - Survey Research - Case Study Research.
Data Collection: - Sources of Data: Primary Data, Secondary Data; Procedure Questionnaire - Sampling Merits and Demerits - Experiments - Kinds - Procedure; Control Observation - Merits - Demerits - Kinds - Procedure - Sampling Errors - Type-I Error - Type-II Error.
Research Reports: Structure and Components of Research Report, Types of Report, Good Research Report, Pictures and Graphs.
Quantitative Methods : Meaning and definition of quantitative method, Arranging data to convey meaning – Tables, Graphs and Frequency, Distribution, Measures of Central Tendency and Dispersion, Simple and Multiple regression and correlation, Association of Attributes, Probability – probability Distributions, Binomial, Poisson and Normal, Linear Programming - Formulation and Graphical solution to two, variables – Assignment Problems, Transportation problems, Queuing Theory – Single Server And Multi-Server
Markov chains with Simulation Techniques – Monte Carlo Simulation, Game theory – 2x2 zero sum game with dominance – pure strategy and mixed strategy, Decision Theory – 5 criteria of decision making, Chi-square, Discriminant Analysis, Factor Analysis, Cluster Analysis, Multidimensional Scaling, ‘T’ Test, ‘F’ Test, ‘Z’ Test
Introduction : Classification of computers, computer memory, types of software’s: application of system software’s operating systems and types, single user, multi user, multi-tasking single tasking, application of computer for business and research.
Data Communication and networks : Data communication concepts, local area network, wide area network, internet, intranet, extranet, website. Email, search engines-enterprise E communication and E collaboration
MS Office and its application : File handling in window, various versions of MS Office, M S-Word: Test formatting, Mail merge, Macro, M S-Excel: Features, various formulas and functions M.S. Power Point: Creating presentations and adding effects.
SPSS : Introduction to SPSS: Definition, objectives and features, data analysis using SPSS: Data entry creating variables, switching to data labels, data analysis: Frequencies, recording into different variables, cross tabulations and layers.
Application of Internet in research : INFLIBNET, Use of Internet, sights (DOAJ), Use of E Journals, Use of E library, use of EBSCO HOST online database of Academic Libraries.
Examining the methods of evaluating and interpreting published Research, Developing skills needed to research available literature for information relevant to a given topic, Exploring the principles and techniques of topic/project development and testing.
Examining the methods of evaluating and interpreting data collected in the research process, Developing an understanding of the various statistical methods that can be used to analyze data.
Demonstrating the ability to use statistical analysis tools and apply them in decision making activities, Demonstrating the ability to create a template document that can be used to complete your research project or thesis.
Paper writing and report generation : Basic concept of Paper / Thesis writing and report generation, writing Research Abstract, Introduction, review of literature, Result, Conclusion, Concepts of Bibliography and References; significance of report writing, steps of report writing, Types of Research reports. Methods of presentation of report. Formats of publication in research general.
The course work classes are mandatory to attend.
The assignment should be submitted time to time.
Evaluation examinations will be held in the end of course work.
UNIT - 1
Microbial growth and growth kinetics: Bacterial growth curve, generation time, measurement of microbial growth, growth kinetics, synchronous culture, continuous and batch culture, chemostat and turbidostat, environmental factors affecting growth, nutritional diversity in bacteria
Solute transport – Active and passive transport,Primary and secondary transport, Transport kinetics, ABC transporter, PEP-PTS system, catabolite repression, inducer expulsion
UNIT - 2
Diversity and regulation of glucose metabolism in microbes – Embden-Meyerhof- Parnas pathway - Variations of EMP pathway in different groups of bacteria; Overall balance sheet; Regulation; Modes of NAD regeneration; alcoholic and lactic acid fermentation, Pentose phosphate pathway – HMP pathway and its link with glycolysis, Fermentative mode of glucose oxidation - Entner-Doudoroff pathway; variations of ED pathway in different groups of microbes and its implications, Fate of pyruvate, Citric acid pathway – Stoichiometry and energy gain; Regulation; Alternate forms of TCA; Reductive TCA; Branched TCA; Glyoxylate cycle
UNIT - 3
Nitrogen metabolism - Nitrogen assimilation, GS-GOGAT pathway and its regulation, Utilization of other modes of nitrogen, nitrate and nitrite utilization, amino acid biosynthetic pathways and their regulation, amino acid utilization – reduction amination and deamination; decarboxylation; Stickland reaction; amino acid oxidases, polyamine biosynthesis and utilization
UNIT - 4
Lipid metabolism – Biochemistry of lipids, lipid distribution in different groups of microbes,fatty acid biosynthesis, synthesiws of different types of lipids – neutral lipids; phospholipids and glycolipids, biosynthesis of archaeal lipids, synthesis of storage lipids, lipid utilization, beta-oxidation pathway – regulation and energy calculation, Lipid accumulation pathway, biochemical and molecular distinction between oleaginous and non-oleaginous microbes
UNIT - 5
Programming metabolism in relation to overproduction of selected metabolites -
Introduction to primary and secondary metabolism, classification of secondary metabolites, introduction to metabolic engineering – strain development and pathway engineering, Case studies on primary metabolites viz. citric acid, succinic acid, lactic acid, ethanol fermentation, amino acid pathways (glutamate, lysine, shikimic acid), Case studies on secondary metabolites viz. polyhydroxyalkanoates, polyketides and antibiotics
Study material and reference books:
1. “Physiology and Biochemistry of Prokaryotes” by David White, published by Oxford University Press, 4th edition, 2011
2. “Microbial Biochemistry” by G. N. Cohen published by Springer Netherlands, 3rd edition, 2014
3. “Microbial Physiology” by Albert G. Moat, John W. Foster, Michael P. Spector, published by John Wiley & Sons, 4th edition, 2002
4. “Biochemistry” by Geoffrey Zubay, published by William C Brown, 4th edition, 2002
5. “The Metabolic Pathway Engineering Handbook” by Christina Smolke, published by CRC Press, 2009